Fans commented that the usually-flawless "Material Girl" singer looked "plastic" and "unrecognizable" as she gave a speech at the Chime For Change female empowering event held in London, with her face looking more taut than normal.
Dr. Giorgio Netri from Transform Cosmetic Surgery in the U.K. believes Madonna's strange face is the result of "too much" Botox and dermal fillers.
"This isn't a great look," he told UK magazine Look. "It seems to me she has too much product in her face and needs to lay off the treatments for six months."
Dr. Netri thinks that the 54-year-old has an excess amount of face-freezing Botox injected into the frown line, forehead and around the eyes. He also suggests she may have also had other treatments in the past.
"There's some slight puckering close to her jaw line, which may be a sign of a contour thread lift, and this can last a couple of years. The surface of her skin also looks flawless -- this may be down to some kind of skin surfacing treatment," he analysed, before offering some hope.
"Things may settle down over the coming weeks, giving her a much better result."
Despite the plastic surgery rumours, there's no denying that Madge looks pretty great for her age.
Scroll through the gallery above to see Madonna's transformation over the years and tell us what you think of her look.